1 - Select your donor blocks |
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2 - Setup your tissue array organisation. MTA designer software simplifies this operation (see next section) |
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3 - Define punching zone on all donor blocks.
Either using the block itself or a stained slide prepared from the block |
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4 - Set the micrometers to the first position on the recipient block according to your plan. |
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5- Make the first recipient block hole and discard the paraffin core. |
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6 - Place the donor block under the donor punch and sample the tissue from the pre-defined zone. |
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7 - Transfer the tissue core into the hole in the recipient block |
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8 - Repeat the procedure for all positions on your experimental plan |
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9 - Once completed gently heat the block to fix the cores |
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10 - Mount the block in the microtome, cut, mount, stain and analyse your array |